Tips for Proper Vial Calibration for Accurate and Precise Quantitative Analysis
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Pipette Calibration and Standards - Sartorius
Pipette calibration consists of:- Checking the accuracy and precision of the pipette using 4 – 10 measurements per volume at 1 – 4 different volumes (usually selected from minimum or 10%, 50%, and 100% of -the nominal volume of the pipette) - Calculation of results and comparison against pass/fail limits Issuing of a calibration certificate.
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Pipetting Technique | Good Pipetting Practice | Pipetting
Greater accuracy and sample-to-sample reproducibility can be achieved by ensuring that the last remaining droplet dispenses fully and does not adhere to the tip end. For most applications, dispensing with the end of the tip resting against the vessel wall is recommended as it reduces or eliminates the amount of sample remaining in the tip.
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Analytical Balances and Proper Weighing Practices
After using the weights, place them inside a desiccator to keep them dry. 5. Take the right measures to weigh the samples. Use a clean spatula of appropriate size while placing the sample. Weigh the sample quantity in a volumetric flask rather than opting for butter paper weighing. The latter can introduce errors.
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2.1: Using micropipettes correctly - Biology LibreTexts
Sep 13, 2022 · Accuracy and precision. Accuracy depends on the micropipette delivering the correct volume. Precise results are reproducible. Let’s use a target analogy to demonstrate the difference between accurate and precise results. Imagine that four students try to hit the bulls-eye five times. Students A and B are precise, while students A and C are
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Performance assessment of a NaI(Tl) gamma counter for PET
May 6, 2015 · A characteristic feature of positron emitters meant that accurate calibration could be maintained over a wide range of sample volumes by using a narrow energy window centered on the 511-keV peak. Although NaI(Tl) gamma counters play an important role in many quantitative positron emission tomography (PET) protocols, their calibration for
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Guide to Ultra-Performance Liquid Chromatography (UPLC) | Waters
The ACQUITY UPLC System [Figure 1] has removed those barriers, enabling columns packed with smaller particles [1.7–1.8 µm] to reach their theoretical performance, while precisely delivering mobile phase at pressures up to 1030 bar [15,000 psi], thus providing a new level of chromatographic performance. UPLC Technology facilitates
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Calibration methods in qualitative analysis - ScienceDirect
May 1, 2022 · Fig. 1. Analytical calibration in qualitative analysis: analyte of type b 0 is identified as of type b x on the basis of model function, Y = G (b), matched to real function, Y = F (b), and of signal value Y 0 measured for a sample. In this situation, the analyst's role is to formulate an auxiliary model function, Y = G (b), whose task is to
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Eight Steps to Method Validation in a Clinical Diagnostic
characteristics such as the sensitivity, specificity, accuracy, precision, reportable range, and performance standards. Performing Data Analysis The test result values (data) obtained from the Y hospital laboratory and the X reference laboratory are plotted on graphs and observed for any outliers. The mean, SD, and CV are
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METHOD 3815 SCREENING SOLID SAMPLES FOR VOLATILE ORGANICS
2.2 The sample plug is immediately extruded into a glass vial containing organic-free reagent water. The vial is immediately covered with aluminum foil and a modified septum cap. 2.3 The sealed vial containing the sample is hand-shaken for 10 - 15 sec and the sample is visually examined to ensure that complete dispersion of the sample in the
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Volume Determination - Chemistry LibreTexts
Aug 20, 2019 · Introduction. Chemists use two general methods to measure exact quantities of chemical reagents, mass determination by using chemical balances and volume determination of liquids and solutions by using calibrated glassware. Volumes can be crudely determined using the calibration markings on some beakers and flasks.
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1.5: Calibration of Instrumental Methods - Chemistry LibreTexts
Jan 22, 2023 · The exact standardization, or calibration relationship, is determined by an appropriate curve-fitting algorithm. Linear regression, which also is known as the method of least squares, is one such algorithm. Its use is covered in Appendix 1. There are two advantages to a multiple-point standardization.
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Calibration and Linear Regression Analysis: A Self-Guided
Part 2 – The Calibration Curve, Correlation Coefficient and Confidence Limits CHM314 Instrumental Analysis Department of Chemistry, University of Toronto Dr. D. Stone (prepared by J. Ellis) 1 The Calibration Curve and Correlation Coefficient Every instrument used in chemical analysis can be characterised by a specific response function, that is
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An Introduction to Standards and Quality Control for the
analyte and can be used to assess accuracy. Precision determines the consistency, or repeatability, of analytical measurements. Testing standard solutions may aid in the improvement of precision and accuracy of chemical analyses. Refer to Figure 1 for examples of accuracy and precision.
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Learning the basics – how to work with volumetric instruments
From raw material to finished precision instrument Blanks for graduated cylinders Calibration Calibration of graduated pipettes Calibration 'to deliver' (TD, Ex): The delivered quantity of liquid corresponds to the capacity printed on the instrument. The wetting residue remaining in the instrument has already been taken into account in the
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DRAFT GUIDANCE DOCUMENT ON SINGLE LABORATORY - OECD
accurate calibration is to be maintained should be submitted. Where a non-linear calibration is used, an explanation (including how calibration accuracy is to be maintained) is provided. c) Accuracy (Recovery) – The determination of accuracy for the active substance in the technical material is not required.
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